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Techniques & Equipment

The Maryanovich Lab uses a wide array of techniques, including confocal microscopy, flow cytometry, cell sorting, and more!

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Confocal Microscopy of the HSC niche

The Maryanovich Lab uses confocal microscopy to image the HSC niche, including stromal cells, endothelial cells, & nerves. The relationship of these cells to HSCs is critical in maintaining a supportive environment. Alterations in the niche create conditions that support cancer development and aging of HSCs

Flow Cytometry & Cell Sorting

Flow cytometry and cell sorting are staples of the lab. These techniques quantify the numbers of hematopoietic and supporting niche cells isolated from bone marrow and other tissues. They also allow us to isolate specific cell types for downstream applications, such as RT-qPCR, bulk sequencing, proteomics, etc.

Circadian Studies

HSCs and the bone marrow niche are regulated by circadian oscillations via the sympathetic nervous system. We implement light cyclers to house mice where the onset of light & dark occur at different times of day. This allows us to isolate cells at different circadian times for experimentation.

Mouse Surgery & Transplantation

The study of HSCs, including their mobilization & function, requires the implementation of advanced mouse surgical, transplantation, & dissection techniques. Our lab performs hands-on training for each member to become proficient in these techniques, providing consistency across experiments.

Leica CM3050s Cryostat

Histology is a core technique of the Maryanovich lab. We have access to this Leica cryostat for generating cryosections of hematopoietic tissues. We use the Kawamoto tape method for cryosectioning of non-decalcified femoral bone marrow in addition to standard sectioning of soft tissues for standard and confocal microscopy.